Design of genome-scale oligonucleotide-based probes for fluorescence in situ hybridization (FISH)



Chorus2, a software that is developed specifically for oligo selection in plant species. It is highly effective to remove all repetitive elements in selection of single copy oligos, which is critical for the development of successful FISH probes. Chorus2 is more effective than Chorus and OligoMiner for repeat removal. Chorus2 allows to select oligos that are conserved among related species, which extends the usage of oligo-FISH probes among phylogenetically related plant species. Chorus2 allows development of FISH probes from plant species without an assembled genome.

Video tutorials

YouTube (EN)
bilibili (中文)

Oligo design with Chorus/Chorus2

Oligo-FISH performed with Chorus/Chorus2 designed probes

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Oligo database

We have developed an oligo database for several plant species. The database currently covers six popular plant species.
The oligo database is available here.