CRISPR-BETS

CRISPR-BETS

A gRNA design software for base editing knockout using stop codon.

Web Server

Desktop Download (Please refer to the documentation for installation)

Documentation

Github / Gitee

Video tutorial

Introduction

CRISPR-BETS (CRISPR-Base Editing To Stop codon) is developed for designing gRNA to knockout gene by CRISPR base editing system. CRISPR-BETS has both online and desktop version, which are integrated into a user-friendly graphic user interface (GUI) and compatible with major operating systems (Windows, MacOS, Linux). Guide RNAs can be designed easily without any environment installation. Users can complete the analysis with a simple mouse click. Results will display with detailed information and fancy images, which are able to be downloaded. Moreover, CRISPR-BETS allows instant check the gRNA specificity.

Preview

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Citation

Wu YC†, He Y†, Sretenovic S, Liu SS, Cheng YH, Han YS, Liu GQ, Bao Y, Fang Q, Zheng XL, Zhou JP, Qi YP*, Zhang Y*, Zhang T*. CRISPR-BETS: A base editing design tool for generating stop codons. Plant Biotechnology Journal 2022, 20(3):499-510

Han YS†, Liu GQ†, Wu YC, Bao Y, Zhang Y*, Zhang T*.CrisprStitch: Fast evaluation of the efficiency of CRISPR editing systems. Plant Communications DOI: https://doi.org/10.1016/j.xplc.2023.100783